文章摘要
胡俊茹,王国霞,孙育平,赵红霞,黄燕华,曹俊明.亚硒酸钠和酵母硒对黄颡鱼幼鱼生长性能、抗氧化能力及抗低温应激的影响[J].水产学报,2019,43(11):2394~2404
亚硒酸钠和酵母硒对黄颡鱼幼鱼生长性能、抗氧化能力及抗低温应激的影响
Effects of dietary sodium selenite and selenoyeast on growth performance, antioxidant responses and low temperature stress resistance of juvenile yellow catfish (Pelteobagrus fulvidraco)
投稿时间:2018-10-10  修订日期:2018-12-15
DOI:10.11964/jfc.20181011479
中文关键词: 黄颡鱼    生长性能  抗氧化能力  抗低温应激
英文关键词: Pelteobagrus fulvidraco  Se  growth performance  antioxidant responses  low temperature stress
基金项目:广东省自然科学基金(2018A030313501);华南师范大学重点科技平台开放课题
作者单位E-mail
胡俊茹 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640
广州飞禧特生物科技有限公司, 广东 广州 510640
华南师范大学, 广东省水产健康安全养殖重点实验室, 广东 广州 510631 
 
王国霞 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640
广州飞禧特生物科技有限公司, 广东 广州 510640 
 
孙育平 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640
广州飞禧特生物科技有限公司, 广东 广州 510640 
 
赵红霞 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640
广州飞禧特生物科技有限公司, 广东 广州 510640 
 
黄燕华 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640
广州飞禧特生物科技有限公司, 广东 广州 510640 
 
曹俊明 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东 广州 510640 junmcao@163.com 
摘要点击次数: 51
全文下载次数: 68
中文摘要:
      本实验旨在研究饲料中添加亚硒酸钠和酵母硒对黄颡鱼幼鱼生长性能、抗氧化能力及抗低温应激的影响。实验选用初始体质量为(2.12±0.01) g的黄颡鱼幼鱼为研究对象,随机分为4组,每组3个重复,分别投喂含硒0.028 mg/kg (对照组)、0.25 mg/kg (亚硒酸钠)、0.30 mg/kg (酵母硒)和0.52 mg/kg (酵母硒)的实验饲料,记为G0、G1、G2和G3组,养殖56 d。养殖结束后,进行低温应激实验。结果显示,亚硒酸钠和酵母硒的添加对幼鱼的终末均重、增重率、存活率和饲料系数影响不显著。亚硒酸钠和酵母硒显著提高全鱼和肌肉的硒含量,G3组显著高于其他组,G1与G2组全鱼硒含量差异不显著,但G2组肌肉硒含量显著高于G1组。亚硒酸钠和酵母硒显著提高了幼鱼肝脏GPx活性,G1和G2组SOD活性显著高于G0组,G3组与G0组差异不显著。22 ℃时,各组间血浆总蛋白、胆固醇、甘油三酯和尿素氮含量无显著差异,但G2组血糖含量显著高于G0和G1组,与G3组差异不显著,各组HSP70 mRNA表达量无显著差异。13 ℃时,各组黄颡鱼血浆总蛋白、胆固醇含量无显著差异,但G1组甘油三酯含量显著高于G0和G3组,与G2组差异不显著;G2和G3组血糖含量显著高于G0和G1组;G2组血浆尿素氮含量显著低于G1组,与其他组差异不显著;G0组HSP70 mRNA相对表达量显著高于其他组。研究表明,在综合生长性能、全鱼和肌肉硒沉积、肝脏抗氧化能力以及鱼体抗低温应激效果方面,饲料中添加0.30 mg/kg酵母硒效果优于0.25 mg/kg亚硒酸钠。
英文摘要:
      In order to investigate the effects of dietary sodium selenite and selenoyeast on growth performance, antioxidant responses and low temperature stress resistance of juvenile yellow catfish Pelteobagrus fulvidraco, four isonitrogenous and isolipid diets containing graded levels of Se, 0.028 (control, G0), 0.25 (sodium selenite, G1), 0.30 (selenoyeast, G2), 0.52 (selenoyeast, G3) mg/kg were randomly fed to P. fulvidraco [initial weight (2.12±0.01) g] for 56 days. At the end of breeding experiment, the low temperature stress test was conducted. The results showed that sodium selenite and selenoyeast had no significant effects on the final weight, weight gain rate, feed coefficient and survival rate. Sodium selenite and selenium yeast significantly increased the selenium content of whole body and muscle, and G3 group was significantly higher. The selenium content of whole body had no significant difference between G1 and G2 groups, but the selenium content of muscle in G2 group was significantly higher than that of G1 group. Sodium selenite and selenium yeast significantly increased GPx activity in liver. In G1 and G2 groups, SOD activity was significantly higher than that in G0 group. At 22 ℃, the total protein, cholesterol, triglycerides, and urea nitrogen contents had no significant difference among all the groups, but G2 group had significantly higher glucose than G0 and G1 groups. The relative expression of HSP70 mRNA had no significant difference. At 13 ℃, the total protein, cholesterol content had no significant difference. The triglyceride content in G1 group was significantly higher than those in G0 and G3 groups, but was not significantly different compared to G2 group. Glucose in G2 and G3 groups were significantly higher than those in G0 and G1 groups. The urea nitrogen content in G2 group was significantly lower than that in G1 group. The relative expression of HSP70 mRNA in G0 group was significantly higher than those in other groups. The results showed that 0.30 mg/kg selenoyeast in juvenile P. fulvidraco diet was better than 0.25 mg/kg sodium selenite, taken the growth performance, selenium deposition, antioxidant activity and low temperature stress resistance into consideration.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看